"The trypsin tips from ProteoGen Bio have proven to be an outstanding tool in our sample preparation for mass spectometry. The tryptic digest is effective and rapid, and potential sample loss is minimized since no subsequent clean-up step is necessary. The ammonium bicarbonate buffer from the tryptic digestion can simply be removed by centrifugal evaporation."
Albrecht Moritz Ph.D.
Cell Signaling Technology
"In our laboratory activity, we used Digestips to develop a quantitative LC/MS methods, in serum, for the detection of recombinant proteins. Despite the complexity of the matrix, the easy digestion procedure coupled with the extreme results reproducibility allowed us to successfully use this technology where traditional in-solution methods failed."
Dr. Luca Barbero
Istituto di Ricerche Biomediche "A. Marxer" - Merck Serono
"We have successfully applied DigesTip trypsin and chymotrypsin for proteolysis of small proteins (up to 10 kDa) to determine de novo their entire amino acid sequence. The tips are easy to handle and exhibit a specific and high cleavage efficiency. We applied our protocols to less than 100ng of protein obtaining a reliable digestion."
Urs Kämpfer
Laboratory of Protein Analysis (Prof. J. Schaller)
Department of Chemistry and Biochemistry - University of Bern
Department of Chemistry and Biochemistry - University of Bern
"We use DigesTip for sample digestions in resolving little complex samples within little amount of total protein. In these experiments the digestions come out correctly, obtaining good MALDI spectra and MS/MS spectra and good identifications of proteins present in the sample, using as a search engine MASCOT and Sequest,respectively. DigesTip is a quick and easy solution to process samples with good results in the identification of peptides. The results are comparable to those obtained with standard methods and are quite reproducible."
Dra. Esperanza Morato López
Proteomics Facility
Centro de Biología Molecular Severo Ochoa, CSIC - Universidad Autonoma de Madrid
Centro de Biología Molecular Severo Ochoa, CSIC - Universidad Autonoma de Madrid




