DigesTip trypsin is a micro-bioreactor shaped as a pipette tip with a filter containing immobilized trypsin.
DigesTip trypsin exhibits unprecedented great performance in terms of ease of use, digestion time and quality of results. It is both a powerful resource for tryptic digestion and a first choice to highly improve the productivity of any Mass Spectrometry lab. Available in two different formats, DigesTip trypsin is compatible with the majority of 20 µl and 200 µl single-channel and multi-channel pipettors, including: Gilson, Biohit, Rainin, Eppendorf, Brand.
DigesTip trypsin exhibits unprecedented great performance in terms of ease of use, digestion time and quality of results. It is both a powerful resource for tryptic digestion and a first choice to highly improve the productivity of any Mass Spectrometry lab. Available in two different formats, DigesTip trypsin is compatible with the majority of 20 µl and 200 µl single-channel and multi-channel pipettors, including: Gilson, Biohit, Rainin, Eppendorf, Brand.
HSA
DigesTip preparation time: Not required
Digestion time: 1 minute
Spectrum obtained by digesting with DigesTip trypsin at room temperature for exactly 1 minute, 2 µl of a solution of reduced and alkylated HSA at the concentration of 5 µg/ml. Digested sample was analysed by MALDI-TOF MS, obtaining 74% of coverage
DigesTip preparation time: Not required
Digestion time: 1 minute
Spectrum obtained by digesting with DigesTip trypsin at room temperature for exactly 1 minute, 2 µl of a solution of reduced and alkylated HSA at the concentration of 5 µg/ml. Digested sample was analysed by MALDI-TOF MS, obtaining 74% of coverage
HSA matched peptides with no missed cleavage sites
Protein mixture
DigesTip preparation time: Not required
Digestion time: 1 minute
Spectrum obtained by digesting with DigesTip trypsin at room temperature for exactly 1 minute a reduced and alkylated five-protein mixture (lysozyme, carbonic anhydrase, fumarase, BSA, B-Galactosidase). Digested sample was analyzed by MALDI-TOF MS.
DigesTip preparation time: Not required
Digestion time: 1 minute
Spectrum obtained by digesting with DigesTip trypsin at room temperature for exactly 1 minute a reduced and alkylated five-protein mixture (lysozyme, carbonic anhydrase, fumarase, BSA, B-Galactosidase). Digested sample was analyzed by MALDI-TOF MS.
Coverage and number of matched peptides for each protein within the mixture
DigesTip trypsin10 µl is proven for use on a variety of automated liquid handling systems including:
- Tecan FreedomEVO Workstation
- Genomic Solutions ProMS MALDI Preparation Station
- Genomic Solutions ProPrep Protein Digestion and Mass Spec Preparation System
- Gilson Quad-Z 215 Liquid Handler with Disposable Tips
- Perkin Elmer TOFPrep MALDI Spotting System
- CyBio CyBi - Well 96 Automated Pipettor.
