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DigesTip Trypsin

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DigesTip trypsin is a micro-bioreactor shaped as a pipette tip with a filter containing immobilized trypsin. DigesTip trypsin exhibits unprecedented great performance in terms of ease of use, digestion time and quality of results. It is both a powerful resource for tryptic digestion and a first choice to highly improve the productivity of any Mass Spectrometry lab. Available in two different formats, DigesTip trypsin is compatible with the majority of 20 μl and 200 μl single-channel and multi-channel pipettors, including Gilson, Rainin, Eppendorf, Biohit, Brand.

High quality digestion and reduced trypsin autolysis DigesTip trypsin performs high quality tryptic digestion with low quantities of samples (up to 10 ng), a considerable reduction of trypsin autolysis and the absence of contaminations.
Reduced tryptic digestion down to 1 minute

With DigesTip trypsin, 1 minute is enough! Aspirate the sample to be digested and press and release the plunger repeatedly for one minute.
Verify the result with mass spectrometry: a clear high-coverage spectrum will be obtained!

Usable by robots for high throughput proteomics A lab-automation robot can do this! DigesTip trypsin, being proven for use on a variety of automated liquid handling systems, can be used in automated high-throughput proteomics pipelines.

 

No preparation step before use and no additional clean-up Unlike other similar products, DigesTip trypsin does not require any preparation phase or any subsequent purification step.

Technical Facts

Protein mixture
Spectrum obtained by digesting with DigesTip trypsin at room temperature for exactly 1 minute a reduced and alkylated five-protein mixture (lysozyme, carbonic anhydrase, fumarase, BSA, B-Galactosidase). Digested sample was analyzed by MALDI-TOF MS.
Coverage and number of matched peptides for each protein within the mixture
Protein mixture
Usable by robots
DigesTip trypsin 10 μl is proven for use on a variety of automated liquid handling systems including:
  • Tecan FreedomEVO Workstation
  • Genomic Solutions ProMS MALDI Preparation Station
  • Genomic Solutions ProPrep Protein Digestion and Mass Spec Preparation System
  • Gilson Quad-Z 215 Liquid Handler with Disposable Tips
  • Perkin Elmer TOFPrep MALDI Spotting System
  • CyBio CyBi - Well 96 Automated Pipettor.
DigesTip trypsin can improve the performance of an automated high-throughput proteomics pipeline, adding value to your investment.
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