DigesTip glu-C

Product Values

DigesTip trypsin

DigesTip chymotrypsin

DigesTip glu-C

DigesTip papain

DigesTip proteinase K

Immobilization on Demand

Site Map

Seminars

Protocol Video

Order Now

DigesTip glu-C is a micro-bioreactor shaped as a pipette tip containing immobilized endoproteinase Glu-C. DigesTip glu-C exhibits great performance in terms of ease of use, digestion time and quality of results. It is both a powerful resource for endoproteinase Glu-C digestion and a first choice to highly improve the productivity of any Mass Spectrometry lab. DigesTip glu-C is compatible with the majority of 20 μl and 200 μl single-channel and multi-channel pipettors, including Gilson, Rainin, Eppendorf, Biohit, Brand.

	Improve digestion quality: DigesTip glu-C delivers excellent results with small samples (as small as 40 ng), a considerable reduction of endoproteinase Glu-C autolysis and absence of enzyme contamination.
	DigesTip Glu-C gives you back your valuable time. Protein digestion usually requires several hours. With DigesTip glu-C, 2 minutes are enough. All that is required is to pipette the sample to be digested, pressing and releasing the plunger repeatedly for 2 minutes, to obtain a clear high-coverage spectrum.
	A lab-automation robot can do this! DigesTip glu-C , being proven for use on a variety of automated liquid handling systems, can be used in automated high-throughput proteomics pipelines.
	Unlike conventional products, DigesTip glu-C does not require any preparation phase or any subsequent purification step.

Technical Facts

Spectrum obtained by digesting with DigesTip glu-C, for exactly 3 minutes at room temperature, a three-protein mixture (Human Transferrin, BSA and ß-Casein from bovine milk), previously denatured and reduced for 10 minutes in 40 mM ammonium bicarbonate, pH 7.8 + 10% acetonitrile + 50 mM DTT, without the need to alkylate the sample before digestion. Digested samples were analysed by MALDI-TOF MS.

DigesTip glu-C 10 μl is proven for use on a variety of automated liquid handling systems including:

Tecan FreedomEVO Workstation
Genomic Solutions ProMS MALDI Preparation Station
Genomic Solutions ProPrep Protein Digestion and Mass Spec Preparation System
Gilson Quad-Z 215 Liquid Handler with Disposable Tips
Perkin Elmer TOFPrep MALDI Spotting System
CyBio CyBi - Well 96 Automated Pipettor.

DigesTip glu-C can improve the performance of an automated high-throughput proteomics pipeline, adding value to your investment.