![]() |
Improve digestion quality: DigesTip papain delivers excellent results in Fab fragment generation (up to 20 µg of antibodies per DigesTip) and guarantees the absence of enzyme contamination. |
![]() |
DigesTip papain gives you back your valuable time. Antibody/protein digestion usually requires several hours. With DigesTip papain, 1 minute is enough. All that is required is to pipette the sample to be digested, pressing and releasing the plunger repeatedly for 1 minute, to obtain Fab fragments or peptide mixtures. |
![]() |
A lab-automation robot can do this! DigesTip papain, being proven for use on a variety of automated liquid handling systems, can be used in automated high-throughput proteomics pipelines. |
![]() |
Unlike conventional products, DigesTip papain allows to easily control the antibody fragmentation by simply removing the DigesTip from the sample to stop the digestion. |
Technical Facts
Lane B: In-solution digestion, 7 hours at 37°C.
Lane C: 1-minute digestion with DigesTip Papain.
Lane D: 3-minute digestion with DigesTip Papain.
Lane E: 5-minute digestion with DigesTip Papain.
Lane F: Molecular Weight Standard.

SDS-PAGE of DigesTip-digested antibodies, at 3 different times of digestion. The amount of Fab fragments (30 kDa in reducing SDS-PAGE) is consistent after only 1 minute of digestion, and rises as the digestion time is increased. After only 5 minutes, results are comparable with those of time consuming in-solution digestion.

DigesTip papain 10 μl is proven for use on a variety of automated liquid handling systems including:
- Tecan FreedomEVO Workstation
- Genomic Solutions ProMS MALDI Preparation Station
- Genomic Solutions ProPrep Protein Digestion and Mass Spec Preparation System
- Gilson Quad-Z 215 Liquid Handler with Disposable Tips
- Perkin Elmer TOFPrep MALDI Spotting System
- CyBio CyBi - Well 96 Automated Pipettor.
DigesTip papain can improve the performance of an automated high-throughput proteomics pipeline, adding value to your investment.






