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DigesTip papain is a micro-bioreactor shaped as a pipette tip containing immobilized papain. DigesTip papain exhibits great performance in terms of ease of use, digestion time and quality of results. It is both a powerful resource for Fab generation and protein digestion, and a first choice to highly improve productivity in any procedure requiring papain digestion. DigesTip papain is compatible with the majority of 20 μl and 200 μl single-channel and multi-channel pipettors, including Gilson, Rainin, Eppendorf, Biohit, Brand.

Improve digestion quality: DigesTip papain delivers excellent results in Fab fragment generation (up to 20 µg of antibodies per DigesTip) and guarantees the absence of enzyme contamination. 

DigesTip papain gives you back your valuable time. Antibody/protein digestion usually requires several hours. With DigesTip papain, 1 minute is enough. All that is required is to pipette the sample to be digested, pressing and releasing the plunger repeatedly for 1 minute, to obtain Fab fragments or peptide mixtures. 

A lab-automation robot can do this! DigesTip papain, being proven for use on a variety of automated liquid handling systems, can be used in automated high-throughput proteomics pipelines.

Unlike conventional products, DigesTip papain allows to easily control the antibody fragmentation by simply removing the DigesTip from the sample to stop the digestion.

Technical Facts

Lane A: Undigested antibody.
Lane B: In-solution digestion, 7 hours at 37°C.
Lane C: 1-minute digestion with DigesTip Papain.
Lane D: 3-minute digestion with DigesTip Papain.
Lane E: 5-minute digestion with DigesTip Papain.
Lane F: Molecular Weight Standard.
Reducing SDS-PAGE (coomassie staining) of DigesTip Papain digested rabbit anti-BSA antibodies

SDS-PAGE of DigesTip-digested antibodies, at 3 different times of digestion. The amount of Fab fragments (30 kDa in reducing SDS-PAGE) is consistent after only 1 minute of digestion, and rises as the digestion time is increased. After only 5 minutes, results are comparable with those of time consuming in-solution digestion.

Usable by robots

DigesTip papain 10 μl is proven for use on a variety of automated liquid handling systems including:

  • Tecan FreedomEVO Workstation
  • Genomic Solutions ProMS MALDI Preparation Station
  • Genomic Solutions ProPrep Protein Digestion and Mass Spec Preparation System
  • Gilson Quad-Z 215 Liquid Handler with Disposable Tips
  • Perkin Elmer TOFPrep MALDI Spotting System
  • CyBio CyBi - Well 96 Automated Pipettor.

DigesTip papain can improve the performance of an automated high-throughput proteomics pipeline, adding value to your investment.

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